5th International Workshop

August 19 - 25, 2001. Orthodox Academy, Kolympari, Crete, Greece


Talks are 20 min each; additional time has been scheduled for discussion. Poster previews are 5 min each.

Sunday, August 19

REGISTRATION: 13:00 until late plane group arrives


Monday, August 20

Morning Session: 8:30 - 12:45 (Chairs: P. Shirk, T. Tamura)

8:30-8:45   M. Goldsmith and R. Lecanidou, Introductory Remarks

8:45-8:55   Dr. Alexandros Papaderos: Welcome Address

SESSION 1: Transgenesis

1. T. TAMURA, G.X. Quan, N. Kuwabara and T. Kanda: Improvement of germ-line transformation in the silkworm, Bombyx mori. National Institute of Sericultural and Entomological Science, Tsukuba, Japan

2. J. Thomas, M. Da Rocha, C. Royer, A.-M Grenier, B. MAUCHAMP and G. Chavancy: pB3xP3EGFPaf piggyBac-based vectors allows to reduce tedious screening of Bombyx mori L. transgenic offspring. Unité nationale Séricicole, INRA, La Mulatière, France

3. H. MORI1, S. Sugihara1, M. Yamamoto1, A. Shimo-oka1, and 2D. Jarvis: Transgenesis of the silkworm by use of Autographa californica nucleopolyhedrovirus. 1Dept. Applied Biology, Kyoto Institute of Technology, Kyoto; 2Department of Molecular Biology, U. Wyoming

COFFEE BREAK: 10:10-10:30

4. Ph. FOURNIER, H. Bossin, X. Jousset, P. Barry, P. Cerrutti, C. Lopez, C. Duperray, M. Lopez, and M. Bergoin. Stable transfection and metastable transgene expression of multicopy integrated densovirus derived vectors in the lepidopteran Sf9 cell line. Lab. Pathologie Comparée, INRA-CNRS, St-Christol-lez-Alès; Service de Cytométrie en Flux, INSERM, Montpellier, France

5. Paul D. SHIRK and Hervé Bossin. A densovirus-derived vector for the rapid assessment of promoter activity in insects. USDA ARS Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL

6. H. Fujimoto+*, H. Tani#, Y. Matsuura#, K. Hashido+, K. Tsuchida+, N. Takada+, M. Kobayashi* and H. MAEKAWA+. Evidence for the insertion of the non-LTR retrotransposon,  R2Bm, and an associated 28S rDNA sequence into a specific site of the rDNA locus. +National Institute of Infectious Diseases, *U. Tokyo, #Osaka U.

SHORT BREAK: 11:45–11:55

7. T. ANZAI, H. Takahashi, Y. Kubo, K. Kojima and H. Fujiwara. Functional analysis of TRAS1 retrotransposable element: specific integration into telomere sequence of insects. Dept. of Integrated Biosciences, U. Tokyo

8. M. JINDRA et al. Expression in transformed Bombyx mori driven by a Drosophila heatshock promoter. Institute of Entomology, Czech Academy of Sciences, Ceske Budejovice

GENERAL DISCUSSION: Prospects for Transformation

LUNCH 13:30–14:30

Afternoon Session: 16:00–18:30 (Chairs: P. Couble, K. P. Gopinathan)

SESSION 2: Gene Expression

9. Eric Julien, Marie-Claire Bordeaux, Annie Garel, Jean Claude Prudhomme and Pierre COUBLE. Fork Head alternative binding drives stage-specific gene expression in the silk gland of Bombyx mori. Centre de Génétique Moléculaire et Cellulaire, CNRS, Université Claude Bernard Lyon, Villeurbanne, France

10. S. Sourmeli, L. Kravariti and R. LECANIDOU. In vitro and in vivo analysis of Bombyx early chorion genes. Dept. of Biochemistry and Molecular Biology, University of Athens

11. Lakshmi Srinivasan and Karumathil P. GOPINATHAN. Modulation of tRNA gene expression in Bombyx mori. Dept. of Microbiology and Cell Biology, Indian Institute of Science, Bangalore India

COFFEE BREAK 17:15–17:35


DINNER 19:00–20:00

POSTER SESSION I            20:30–21:15: Formal Viewing of Posters            P1–4, P7–P8

Tuesday, August 21

Morning Sessions: 8:30–12:30 (Chairs: K. Mita, K. Gordon)

SESSION 3: Linkage Mapping and Genomics

12. K. MITA, M. Morimyo, Y. Koike, J. Nohata, M. G. Suzuki, T. Shimada, K. Okano, and S. Maeda. Bombyx genome information. Genome Research Group, Natl. Inst. Radiol. Sci., Chiba, Japan, Dept. of Agri. Environ. Biol., U. Tokyo; RIKEN, Wako, Saitama, Japan

13. W. HARA, E. Nguu, K. Mase, E. Kosegawa and K. Kadono-okuda. 195 ESt-cDNA clones were mapped on 28 chromosomes based on RFLP in the silkworm, Bombyx mori. National Institute of Sericultural and Entomological Science, Tsukuba, Japan

14. J. NAGARAJU, M. Kathirvel, M. Muthulakshmi and M. Dharma Prasad. Mapping and genomic organisation of microsatellite markers in the silkworm, Bombyx mori. Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India

COFFEE BREAK: 9:45–10:05

15. T. SHIMADA1, K. Hamada1,3, N. Omuro1, M. Oami1, M. G. Suzuki1, F. Ohbayashi1, and K. Mita2. Lepidopteran genomes contain peculiar genes that are missing in Drosophila. 1Dept. of Agricultural and Environmental Biology, U. Tokyo, 2National Institute of Agrobiological Sciences, Tsukuba, Japan, 3Present address: Asahi Breweries Co., Ltd.

16. Karl GORDON and Peter East. The lepidopteran midgut and EST databases. CSIRO Entomology Canberra, Australia


17. D. G. HECKEL and L. J. Gahan. Cloning of a gene conferring resistance to Bacillus thuringiensis in Heliothis virescens. Dept. of Genetics, U. Melbourne, Parkville, Victoria, Australia

18. Y. Zheng, S. Zheng, X.-W. Cheng, T. Ladd, P.J. Krell, B. Arif1, A. Retnakaran and Qili FENG. Molecular cloning and insecticidal activity of Choristoneura fumiferana chitinase. Great Lakes Forestry Centre, Canadian Forest Service, Sault Ste. Marie, ON, Canada

SHORT BREAK: 11:20–11:35


LUNCH: 13:00–14:00

Afternoon Sessions: 16:00–18:00 (Chairs: K. Iatrou, R. Lecanidou)

SESSION 5: Genetic Engineering and Cultured Insect Cells

19. D. L. JARVIS and J. R. Hollister. Characterization and genetic engineering of protein glycosylation pathways in lepidopteran insect cells. Department of Molecular Biology, U. Wyoming, Laramie

20. L. Kravariti, L. SWEVERS, M. Xenou-Kokoletsi, V. Mazomenos, N. Ragousis, and K. IATROU. High through-put screening systems for fast detection of biologically active compounds that mimic or antagonize insect growth regulators. Institute of Biology, National Centre for Scientific Research “Demokritos” and Vioryl Chemical Co., Athens, Greece

21. P. Barry, J. Rocher, C. Pelle, S. Gimenez, F. Soler, G. Devauchelle, M. Cerutti and Ph. FOURNIER. Establishment of Spodoptera cell lines involves genome plasticity evidenced by unbalanced polyploidy and chromosome fragmentation. Lab. Pathologie Comparée, INRA-CNRS, Saint-Christol-les-Alès; Lab. Cytogénétique, Faculté de Médecine, Nîmes, France

COFFE BREAK: 17:15–17:35


DINNER 19:00–20:00

POSTER SESSION 2            20:30–21:15: Formal Viewing Of Posters P9–P14, P21-P22

POSTER SESSION 3            21:15–22:00: Formal Viewing Of Posters P15–P20

Wednesday, August 22

Morning Sessions: 8:45–12:00 (Chairs: J. Willis, T. Shimada)

SESSION 6: Sex Determination

22. M. G. SUZUKI1, F. Ohbayashi1, K. Mita2, and T. Shimada1. Structure, sex-specific splicing, and function of Bmdsx, a doublesex homologue of Bombyx mori. 1Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, U. Tokyo; 2National Institute of Agrobiological Sciences, Tsukuba, Japan

23. H. SOTOSHIRO, F. Ohbayashi, M. G. Suzuki, and T. Shimada. Expression of a sex-determining gene, Bmdsx, during sexual differentiation of embryos, fatbody, and gonads in Bombyx mori. Department of Agricultural and Environmental Biology, U. Tokyo

24. F. OHBAYASHI1, M. G. Suzuki1, H. Abe2, N. Tanaka2, T. Yokoyama2, T. Oshiki2, M. Hirokawa3, K. Mita4 and T. Shimada1. Feminizing gene on the W chromosome regulates sex-specific splicing of Bmdsx pre-mRNA in Bombyx mori. 1Dept. of Agricultural and Environmental Biology, U. Toky; 2Tokyo U. of Agriculture and Technology; 3National Institute of Agrobiological Sciences (NIAS), Kobuchizawa, Japan; 4NIAS, Tsukuba, Japan

25. T. NIIMI, H. Oshima, O. Yamashita and T. Yaginuma. Sex determination gene homologues from Bombyx mori: Insight into the evolutionary conservation and divergence. Graduate School of Bioagricultural Sciences, Nagoya U., Nagoya, Japan

COFFEE BREAK: 10:25–10:45

SESSION 7: Wing Development and Gene Expression

26. T. MATSUOKA and H. Fujiwara. Identification of genes abnormally expressed in a wing-deficient mutant (fl) of Bombyx mori. Dept. of Integrated Biosciences, U. Tokyo

27. Manabu OTE1, Kazuei Mita2, Hideki Kawasaki3, Motoaki Seki1, Junko Nohata1, Masataka G. Suzuki1, Masahiko Kobayashi1 and Toru Shimada1.Hormonal regulation of gene expression in wing discs of Bombyx mori during metamorphosis revealed by microarray analysis. 1Department of Agricultural and Environmental Biology, U. Tokyo; 2Genome Research Group, National Institute of Radiological Science, Chiba, Japan; 3Faculty of Agriculture, Utsunomiya U., Utsunomiya, Japan

28. R.H. FFRENCH-CONSTANT and P. B. Koch. Melanism and mimicry in swallowtails: a model for butterfly wing pattern formation. Institute for Zoology I, U. Erlangen-Nürnberg, Erlangen, Germany

LUNCH: 12:45–13:45


 Thursday, August 23

Morning Sessions: 8:30–12:45 (Chairs: T. Trenczek, O. Yamashita)

SESSION 8: Immune Response

29. K. ROXSTROM-LINDQUIST, Y. Assefaw-Redda and Ingrid Faye. Effects of 20-hydroxyecdysone on hemolin gene regulation. Dept. of Genetics, U. Stockholm, Sweden.

30. D. M. LI, U. Theopold and O. Schmidt. Identification of insect coagulation proteins in Galleria mellonella. Department of Applied and Molecular Ecology, Waite Campus, Adelaide U, Glen Osmond, Australia

31. H. SOTOSHIRO, M. Ikeda, and M. Kobayashi. Two unique polypeptides associated with virus-induced cell degeneration in the midgut epithelium of the silkworm, Bombyx mori. Division of Biodynamics, Graduate School of Bioagricultural Science, Nagoya U., Nagoya, Japan.

32. Tina TRENCZEK, Susann Beetz, Traute Holthusen, Tine Lesch and Frank Scholz. Hemocyte changes during infection and late larval development of Manduca sexta. Institute of Zoology, Justus-Liebig University of Giessen, Germany

COFFEE BREAK: 10:10–10:30

33. Tina TRENCZEK, E. Bauer*, K. Ockroy*, A. Viescher*, and S. Dorn.*Pieris brassicae and Cotesia glomerata: hemocyte and hemolymph protein changes during parasitization. Institute of Zoology, Justus-Liebig- U. Giessen; * ETH Zurich, Switzerland

SESSION 9: Neurobiology and Behavior

34. O. Schmidt, M. Beck, A. Reineke, D. LI and U. Theopold. Two co-existing reproductive strategies in a parthenogenetic wasp Venturia canescens. Dept. of Applied and Molecular Ecology, Adelaide U., Glen Osmond, Australia

35. K. SCHELLER, I. Hansen, F. Sehnal. Molecular cloning, synthesis and function of the neuropeptide corazonin in Galleria mellonella. Cell- and Developmental Biology, Biocenter of the University, Wuerzburg, Germany

SHORT BREAK: 11:45–11:55

SESSION 10: Diapause

36. O. YAMASHITA and N. Katagiri. New interests and insights in diapause hormone action in Bombyx mori. Chubu U., Kasugai, Japan and Nagoya U., Nagoya, Japan

37. T. YAGINUMA, T. Niimi and O. Yamashita. Diapause termination in the silkworm, Bombyx mori. Graduate School of Bioagricultural Sciences, Nagoya U., Nagoya, Japan

LUNCH: 12:45–13:45

POSTER SESSION 4            15:00–17:00   All Posters

Friday, August 24

Morning Sessions: 9:00–11:45 (Chairs: L. Guarino, H. Mori)

SESSION 11: Virus Function

38. L. GUARINO. Transcription of heterologous and viral genes by baculovirus RNA polymerase. Depts of Biochemistry and Entomology, Texas A&M U., College Station, TX

39. K. IKEDA, M. Sugiyama, and H. Mori. Cypovirus occlusion into the polyhedra. Dept of Applied Biology, Kyoto Institute of Technology

40. Y. Yamada, G. X. Quan*, T. Kanda*, T. Tamura* S. Asano, K. Sahara and H. BANDO. Inhibition of BmNPV multiplication by the defective mutant of an immediate-early gene product, IE1. Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido U., Sapporo, Japan; *Dept. of Insect Genetics and Breeding, National Institute of Sericultural and Entomological Science, Tsukuba, Japan

COFFEE BREAK: 10:35–10:55


41. Sriramana KANGINAKUDRU1, Chantal Thibert2, Martine Cerutti3, Javare Gowda Nagaraju1, and Pierre Couble2. Stable immunity against baculovirus by means of RNA interference in Sf9 cells. 1Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India; 2Centre de Génétique Moleculaire et Cellulaire, CNRS, Villeurbanne, France; 3Laboratoire de Pathologie Comparée, INRA-CNRS, Saint Christol Lès Alès, France

42. S. G. DEDOS, L. Swevers, and K. Iatrou. Double-stranded RNA mediated inhibition of nuclear receptor expression in a Bombyx mori-derived cell line. Institute of Biology, NCSR “Demokritos,” Athens, Greece

LUNCH: 12:30–13:30

Afternoon Session: 16:00–18:00 (Chairs: J. Rebers, K. Tsuchida)

SESSION 13: Physiology and Biochemistry

43. J. H. WILLIS. Introduction to cuticular protein structure and function. Department of Cellular Biology, U. Georgia, Athens GA

44. J. E. REBERS and J. H. Willis. Interaction of cuticular proteins and chitin. Dept. of Biology, Northern Michigan U., Marquette, MI, and Dept. of Cellular Biology, U. Georgia, Athens, GA

45. M. DHARMA PRASAD1, Won-Jae Lee3, Sung-Jun Han2, Javaregowda Nagaraju1 and Paul T. Brey2. Characterization of a new eukaryotic initiation factor 2 alpha (eIF2a) kinase, BeK, from the silkworm, Bombyx mori. 1Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India; 2Unit de Biochemie et Biologie Moleculaire des Insects, Institut Pasteur, Paris, France; 3Laboratory of Immunology, Center for Medical Science and Research, Yonsei University College of Medicine, Seoul, South Korea

46. A. Fujimori, Y. Okada and M. SUMIDA. Effect of dietary sucrose, glucose or fructose on midgut sucrase activity and midgut protein composition of Bombyx mori in the last fifth instar larvae reared aseptically on an artificial diet. Dept. of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan

COFFE BREAK: 17:35–17:55

GROUP DISCUSSION: Problems and Prospects for Lepidopteran Molecular Biology and Genetics


Saturday, August 25

MORNING SESSION: Evaluation of Workshop Proceedings; Selection of Organizing Committee for VI International Workshop

LUNCH: 12:30–1:30